Difference between revisions of "Part:BBa K3738008"

Latest revision as of 01:52, 18 October 2021

MS2 Bacteriophage Capsid Protein Dimer

The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as well as one copy of maturation protein A (BBa_3738000), which is critical in allowing the capsid to bind to the cell and deliver its cargo. This part is a coding region comprised of 2 copies of the MS2 capsid protein, joined by a histidine tag which is flanked by linker sequences (de Martin Garrido et al., 2020).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 138
Illegal PstI site found at 546
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 138
Illegal PstI site found at 546
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 138
Illegal PstI site found at 546
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 138
Illegal PstI site found at 546
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3738008 short</partinfo>
-The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as well as one copy of maturation protein A (enter part number here), which is critical in allowing the capsid to bind to the cell and deliver its cargo. This part is a coding region comprised of 2 copies of the MS2 capsid protein, joined by a histidine tag which is flanked by linker sequences (de Martin Garrido et al., 2020).
+The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as well as one copy of maturation protein A (BBa_3738000), which is critical in allowing the capsid to bind to the cell and deliver its cargo. This part is a coding region comprised of 2 copies of the MS2 capsid protein, joined by a histidine tag which is flanked by linker sequences (de Martin Garrido et al., 2020).
 <!-- Add more about the biology of this part here