Difference between revisions of "Part:BBa K3738004:Design"
Emily Hagens (Talk | contribs) (→References) |
Emily Hagens (Talk | contribs) (→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| + | This part is a modified version of BBa_K3738000, to which an C-terminal histidine tag and TEV cleavage site have been added. As maturation protein A has not been well studied for use in phage-like particles, we have designed various versions of the part for testing purposes. | ||
| + | |||
This part has been codon optimized for use in E.coli. | This part has been codon optimized for use in E.coli. | ||
| − | |||
| − | |||
===Source=== | ===Source=== | ||
Revision as of 01:43, 18 October 2021
MS2 Bacteriophage Maturation Protein A with C-Terminal Histidine Tag and TEV Cleavage Site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is a modified version of BBa_K3738000, to which an C-terminal histidine tag and TEV cleavage site have been added. As maturation protein A has not been well studied for use in phage-like particles, we have designed various versions of the part for testing purposes.
This part has been codon optimized for use in E.coli.
Source
https://www.uniprot.org/uniprot/P03610
References
Meng, R., Jiang, M., Cui, Z. et al. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nat Commun 10, 3130 (2019). https://doi.org/10.1038/s41467-019-11126-8