Difference between revisions of "Part:BBa K3880008"
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| − | + | CDO1 encodes a cysteine dioxygenase with 190 amino acids which has been found in Streptomyces sp. SCUT-3. CDO1 could catalyze cysteine’s oxidation to produce sulfite, which is the key factor for feather degradation. The previous study have demonstrated that overexpression of cdo1 increased bacterial single-cell sulfite production and feather protein conversion efficiency obviously. | |
| + | Co-overexpression of CDO1 and Sep39 under constitutive promoter ermE increased SCUT-3’s disulfide bond reduction and keratin hydrolysis to achieve higher feather degradation efficiency which exhibited highest total peptide and amino acid yields among SCUT-3, SCUT-Osep39 and SCUT-Ocdo1. However, the co-overexpression of CDO1 and Sep39 under this constitutive promoter ermE yielding fewer cells than SCUT-3 and Sep39 overexpression strain (SCUT-Osep39). To further improve the degradation efficiency without influencing the growth rate, we attempt to modified SCUT-3 through replacing the constitutive promoter with well-characterized promoters. | ||
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Latest revision as of 01:18, 18 October 2021
CDO1(a cysteine dioxygenase)
CDO1 encodes a cysteine dioxygenase with 190 amino acids which has been found in Streptomyces sp. SCUT-3. CDO1 could catalyze cysteine’s oxidation to produce sulfite, which is the key factor for feather degradation. The previous study have demonstrated that overexpression of cdo1 increased bacterial single-cell sulfite production and feather protein conversion efficiency obviously. Co-overexpression of CDO1 and Sep39 under constitutive promoter ermE increased SCUT-3’s disulfide bond reduction and keratin hydrolysis to achieve higher feather degradation efficiency which exhibited highest total peptide and amino acid yields among SCUT-3, SCUT-Osep39 and SCUT-Ocdo1. However, the co-overexpression of CDO1 and Sep39 under this constitutive promoter ermE yielding fewer cells than SCUT-3 and Sep39 overexpression strain (SCUT-Osep39). To further improve the degradation efficiency without influencing the growth rate, we attempt to modified SCUT-3 through replacing the constitutive promoter with well-characterized promoters.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]