Difference between revisions of "Part:BBa P0140"
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<b><h2>Tianjin’s 2021 characterization</h2></b>
 
<b><h2>Tianjin’s 2021 characterization</h2></b>
 
<b><h3>New information of TetR promoter leakage</h3></b>
 
<b><h3>New information of TetR promoter leakage</h3></b>
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<p >Group: Tianjin 2021
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<p >Group: Tianjin 2021<br>
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Author: Ruiqi Liu
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Author: Ruiqi Liu<br>
 
Summary: we added information about TetR promoter leakage.
 
Summary: we added information about TetR promoter leakage.
 
</p>
 
</p>
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[[File:Tianjin-Parts2.jpg|300px|thumb|right|Figure 1.Result]]
 
 
<i><h3>Background</h3></i>
 
<i><h3>Background</h3></i>
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[[File:Tianjin-Parts2.jpg|300px|thumb|right|Figure 1.Result]]
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<p>In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.</p>
 
<p>In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.</p>

Revision as of 00:28, 18 October 2021


PoPS -> TetR [S0163]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


No part name specified with partinfo tag.

Tianjin’s 2021 characterization

New information of TetR promoter leakage

Group: Tianjin 2021
Author: Ruiqi Liu
Summary: we added information about TetR promoter leakage.

Background
Figure 1.Result

In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.

Result

Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results.


Design

Figure 2.Design