Difference between revisions of "Part:BBa K3771077"

 
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<partinfo>BBa_K3771077 short</partinfo>
 
<partinfo>BBa_K3771077 short</partinfo>
  
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<br><b style="font-size:1.3rem">Description</b>
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<br><i>P<sub>trc</sub>-csad</i> is a composite part consisting of the <i>trc</i> promoter and the <i>csad</i> sequences. This part was used in <i>in vivo</i> testing of taurine production. The sequence for <i>csad</i> and <i>trc</i> promoter were ligated and transformed into <i>E. coli</i> to calculate taurine production using high-performance liquid chromatography (HPLC).
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<br><b style="font-size:1.3rem">Biology</b>
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<br><i>trc</i> promoter constitutively facilitates the expression of CSAD enzyme.
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:35%;">
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<p align="center">圖片描述</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/9/92/T--NCKU_Tainan--CSAD2-PCR.png" style="width:35%;">
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<p align="center">Fig. 1 Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/2/26/T--NCKU_Tainan--CSAD2-digestion.png" style="width:35%;">
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</div>
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<p align="center">Fig. 2 Confirmation of  pSUI-Ptrc-CSAD by digestion.
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M: Marker; Lane 1~3: Different colonies of pSUI-Ptrc-CSAD (3674 bp)
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</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/c/cd/T--NCKU_Tainan--CSAD2-plate%28DH5a%29.png" style="width:35%;">
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</div>
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<p align="center">Fig. 3 Transformation/CSAD in DH5α</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/9/92/T--NCKU_Tainan--CSAD-PAGE%28DH5a%29.png" style="width:35%;">
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</div>
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<p align="center">Fig. 4 Confirmation of protein expression of CSAD.
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M: Marker; Lane1: whole cell of CSAD in DH5α; Lane2: soluble protein of CSAD in DH5α (~22 kDa)
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</p>
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<br><b style="font-size:1.3rem">Usage</b>
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<br>
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<br><b style="font-size:1.3rem">Characterization</b>
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<br>
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<br><b style="font-size:1.3rem">References</b>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 21:13, 17 October 2021


Ptrc-CSAD


Description

Ptrc-csad is a composite part consisting of the trc promoter and the csad sequences. This part was used in in vivo testing of taurine production. The sequence for csad and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

trc promoter constitutively facilitates the expression of CSAD enzyme.

圖片描述

Fig. 1 Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)

Fig. 2 Confirmation of pSUI-Ptrc-CSAD by digestion. M: Marker; Lane 1~3: Different colonies of pSUI-Ptrc-CSAD (3674 bp)

Fig. 3 Transformation/CSAD in DH5α

Fig. 4 Confirmation of protein expression of CSAD. M: Marker; Lane1: whole cell of CSAD in DH5α; Lane2: soluble protein of CSAD in DH5α (~22 kDa)


Usage

Characterization

References
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 337
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 122