Difference between revisions of "Part:BBa K3941003:Design"
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===Source===
 
===Source===
  
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The source of this pars is Trichoderma reesei endoglucanase II gene.
+
The source of this part is Trichoderma reesei endoglucanase II gene.
  
 
===References===
 
===References===

Latest revision as of 21:13, 17 October 2021


EGII (D185S)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is created based on only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag was added to 3' end of the sequence to help purification of protein product. A single aminoacid mutation has been studied as Aspartic acid aminoacid in 185 aminoacid position is changed with Serine aminoacid.


Source

The source of this part is Trichoderma reesei endoglucanase II gene.

References