Difference between revisions of "Part:BBa K3776019"
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− | Designed by Vo et al. (2020), this | + | Designed by Vo et al. (2020), this system includes a crRNA which guides the TniQ-Cascade and tnsA-tnsB-tnsC operon to insert a donor DNA into a site in an organism's genome. A single promoter drives the expression of the entire system, and can be switched by users. The 32bp crRNA is seamlessly flanked by two repeats and users should use BsaI restriction enzymes to change the crRNA. This RNA guided protein complex inserts a donor DNA located between two flanks behind the into the genome of the host organism. Users should clone their genes of interest between these two flanks. |
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Revision as of 21:10, 17 October 2021
INTEGRATE system
Designed by Vo et al. (2020), this system includes a crRNA which guides the TniQ-Cascade and tnsA-tnsB-tnsC operon to insert a donor DNA into a site in an organism's genome. A single promoter drives the expression of the entire system, and can be switched by users. The 32bp crRNA is seamlessly flanked by two repeats and users should use BsaI restriction enzymes to change the crRNA. This RNA guided protein complex inserts a donor DNA located between two flanks behind the into the genome of the host organism. Users should clone their genes of interest between these two flanks.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1578
Illegal NheI site found at 3665
Illegal NheI site found at 7783 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1004
Illegal BglII site found at 1795
Illegal BglII site found at 2325
Illegal BglII site found at 7570
Illegal BamHI site found at 147
Illegal BamHI site found at 8698
Illegal XhoI site found at 8681 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 7702
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 113
Illegal BsaI.rc site found at 87
Illegal SapI.rc site found at 3081