Difference between revisions of "Part:BBa K3771075"

 
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<partinfo>BBa_K3771075 short</partinfo>
 
<partinfo>BBa_K3771075 short</partinfo>
  
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<br><b style="font-size:1.3rem">Description</b>
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<br><i>P<sub>trc</sub>-cdo1</i> is a composite part consisting of the <i>trc</i> promoter and the <i>cdo1</i> sequences. This part was used in <i>in vivo</i> testing of taurine production. The sequence for <i>cdo1</i> and <i>trc</i> promoter were ligated and transformed into <i>E. coli</i> to calculate taurine production using high-performance liquid chromatography (HPLC).
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<br><b style="font-size:1.3rem">Biology</b>
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<br>Trc promoter constitutively facilitates the expression of CDO1 enzyme.
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/4/4c/T--NCKU_Tainan--taurine_pathway.png" style="width:35%;">
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</div>
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<p align="center">圖片描述</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/a/ab/T--NCKU_Tainan--CDO1-PCR.png" style="width:35%;">
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<p align="center">Fig. 1 Confirmation of cdo1 fragment by PCR. M: Marker; Lane 1: cdo1 (603 bp)</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/9/9d/T--NCKU_Tainan--CDO1-digestion.png" style="width:35%;">
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<p align="center">Fig. 2 Confirmation of  pSB4KI-Ptrc-CDO1 by digestion.
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M: Marker; Lane 1~3: Different colonies of pSB4KI-Ptrc-CDO1 (5470 bp)</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/9/90/T--NCKU_Tainan--CDO1-plate%28DH5a%29.png" style="width:35%;">
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</div>
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<p align="center">Fig. 3 Transformation / CDO1 in DH5α</p>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/4/4e/T--NCKU_Tainan--CDO1-PAGE.png" style="width:35%;">
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</div>
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<p align="center">Fig. 4 Confirmation of protein expression of CDO1.
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M: Marker; Lane1: CDO1 in DH5α without induction; Lane2: CDO1 in DH5α with induction (~22 kDa)</p>
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<br><b style="font-size:1.3rem">Usage</b>
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<br>
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<br><b style="font-size:1.3rem">Characterization</b>
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<br>
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<br><b style="font-size:1.3rem">References</b>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 20:54, 17 October 2021


Ptrc-CDO1


Description

Ptrc-cdo1 is a composite part consisting of the trc promoter and the cdo1 sequences. This part was used in in vivo testing of taurine production. The sequence for cdo1 and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

Trc promoter constitutively facilitates the expression of CDO1 enzyme.

圖片描述

Fig. 1 Confirmation of cdo1 fragment by PCR. M: Marker; Lane 1: cdo1 (603 bp)

Fig. 2 Confirmation of pSB4KI-Ptrc-CDO1 by digestion. M: Marker; Lane 1~3: Different colonies of pSB4KI-Ptrc-CDO1 (5470 bp)

Fig. 3 Transformation / CDO1 in DH5α

Fig. 4 Confirmation of protein expression of CDO1. M: Marker; Lane1: CDO1 in DH5α without induction; Lane2: CDO1 in DH5α with induction (~22 kDa)


Usage

Characterization

References
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 534
  • 1000
    COMPATIBLE WITH RFC[1000]