Difference between revisions of "Part:BBa K3771032"

 
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<br><b style="font-size:1.3rem">Description</b>
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<br>This composite part is a component of the IFN-γ sensing system and is used to express the taurine production enzyme, JJU10.
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<br><b style="font-size:1.3rem">Biology</b>
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<br>The <i>ompA</i> promoter facilitates the constitutive expression of OmpA/OprF. Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria[1]. Signal transduction from the outer membrane to the inner membrane activates the <i>pspA</i> promoter, initiating expression of JJU10. JJU10 converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.
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<br><b style="font-size:1.3rem">Usage</b>
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<br>We ligased the NPO-OmpA* fragment and pspA-JJU10 on the pSU expression vector and transformed it into DH5alpha to complete construction of the plasmid.
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<br><b style="font-size:1.3rem">Characterization</b>
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<br>Double digestion results are shown in Figure 1.
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<img src="圖片網址" style="width:35%;">
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<p align="center">圖片描述</p>
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<br><b style="font-size:1.3rem">References</b>
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<br>1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x
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<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/</a>
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 19:50, 17 October 2021


PpspA-JJU-PompA-OmpA/OprF


Description

This composite part is a component of the IFN-γ sensing system and is used to express the taurine production enzyme, JJU10.

Biology

The ompA promoter facilitates the constitutive expression of OmpA/OprF. Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria[1]. Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of JJU10. JJU10 converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.

Usage

We ligased the NPO-OmpA* fragment and pspA-JJU10 on the pSU expression vector and transformed it into DH5alpha to complete construction of the plasmid.

Characterization

Double digestion results are shown in Figure 1.

圖片描述


References

1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x https://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features BBa_K3771032 SequenceAndFeatures