Difference between revisions of "Part:BBa K3885203"
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<partinfo>BBa_K3885203 short</partinfo> | <partinfo>BBa_K3885203 short</partinfo> | ||
− | + | The E. coli complex ClpXP, a protease from the AAA+ family, is an ideal system to achieve targeted protein degradation in Cell-Free reactions by adding, either to the N-terminus or C-terminus, a specifific degron typically 10−12 amino acids long.56 In Cell-Free systems,46 as well as in E. coli, 57 protein degradation by ClpXP is a zeroth order reaction with constant rate. <br> | |
− | The E. coli complex ClpXP, a protease from the AAA+ family, is an ideal system to achieve targeted protein degradation in Cell-Free reactions by adding, either to the N-terminus or C-terminus, a specifific degron typically 10−12 amino acids long.56 In Cell-Free systems,46 as well as in E. coli, 57 protein degradation by ClpXP is a zeroth order reaction with constant rate. | + | Under the influence of the P70 promoter, ClpXP protein can be expressed.The expression of ClpXP in Cell-Free system can improve the degradation rate of protein with ssrA degradation label. |
Revision as of 18:17, 17 October 2021
P70-ClpXP
The E. coli complex ClpXP, a protease from the AAA+ family, is an ideal system to achieve targeted protein degradation in Cell-Free reactions by adding, either to the N-terminus or C-terminus, a specifific degron typically 10−12 amino acids long.56 In Cell-Free systems,46 as well as in E. coli, 57 protein degradation by ClpXP is a zeroth order reaction with constant rate.
Under the influence of the P70 promoter, ClpXP protein can be expressed.The expression of ClpXP in Cell-Free system can improve the degradation rate of protein with ssrA degradation label.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 448
Illegal EcoRI site found at 1510
Illegal EcoRI site found at 1855 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 448
Illegal EcoRI site found at 1510
Illegal EcoRI site found at 1855 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 448
Illegal EcoRI site found at 1510
Illegal EcoRI site found at 1855
Illegal BglII site found at 1689 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 448
Illegal EcoRI site found at 1510
Illegal EcoRI site found at 1855 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 448
Illegal EcoRI site found at 1510
Illegal EcoRI site found at 1855
Illegal AgeI site found at 1171
Illegal AgeI site found at 1909 - 1000COMPATIBLE WITH RFC[1000]