Difference between revisions of "Part:BBa K3885203"

 
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<partinfo>BBa_K3885203 short</partinfo>
 
<partinfo>BBa_K3885203 short</partinfo>
  
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Under the influence of the P70 promoter, ClpXP protein can be expressed.
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Under the influence of the P70 promoter, ClpXP protein can be expressed.The expression of ClpXP in Cell-Free system can improve the degradation rate of protein with ssrA degradation label.<br>
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The E. coli complex ClpXP, a protease from the AAA+ family, is an ideal system to achieve targeted protein degradation in Cell-Free reactions by adding, either to the N-terminus or C-terminus, a specifific degron typically 10−12 amino acids long.56 In Cell-Free systems,46 as well as in E. coli, 57 protein degradation by ClpXP is a zeroth order reaction with constant rate.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:17, 17 October 2021


P70-ClpXP

Under the influence of the P70 promoter, ClpXP protein can be expressed.The expression of ClpXP in Cell-Free system can improve the degradation rate of protein with ssrA degradation label.
The E. coli complex ClpXP, a protease from the AAA+ family, is an ideal system to achieve targeted protein degradation in Cell-Free reactions by adding, either to the N-terminus or C-terminus, a specifific degron typically 10−12 amino acids long.56 In Cell-Free systems,46 as well as in E. coli, 57 protein degradation by ClpXP is a zeroth order reaction with constant rate.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1510
    Illegal EcoRI site found at 1855
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1510
    Illegal EcoRI site found at 1855
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1510
    Illegal EcoRI site found at 1855
    Illegal BglII site found at 1689
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1510
    Illegal EcoRI site found at 1855
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1510
    Illegal EcoRI site found at 1855
    Illegal AgeI site found at 1171
    Illegal AgeI site found at 1909
  • 1000
    COMPATIBLE WITH RFC[1000]