Difference between revisions of "Part:BBa K3989005"

 
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Shong, J., Huang, Y. M., Bystroff, C., & Collins, C. H. (2013). Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS chemical biology, 8(4), 789-795.
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A variant of the Quorum Sensing regulator protein EsaR<a href="https://parts.igem.org/Part:BBa_K2116001"> BBa_K2116001 </a> with the Valine at position 220 substituted by Alanine.
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===Characterisation===
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===Usage and Biology===
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According to the literature[1], the amino acid substitution has increased the sensitivity of this protein to 3OC6HSL molecule. The result of our characterisation is shown below and more details can be found in part <a href="https://parts.igem.org/Part:BBa_K3989003"> BBa_K3989003 </a>.
  
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<span class='h3bb'>Sequence and Features</span>
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  <figcaption><b>Figure 1.</b> Fluorescence intensity measurement by plate reader(96-well plate). The measurements were done every one hour and this is the curve of the last test.</figcaption>
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  <img src="https://static.igem.org/mediawiki/parts/9/9f/21_UZurich_characterisation_facs.jpeg">
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  <figcaption><b>Figure 2.</b> Fluorescence intensity measurement by flow cytometry. The samples are taken from the plate, in which the bacteria has been cultured for 7 hours.</figcaption>
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===Sequence and Features===
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<partinfo>BBa_K3989005 SequenceAndFeatures</partinfo>
  
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===References===
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===Functional Parameters===
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<partinfo>BBa_K3989005 parameters</partinfo>
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1) Shong, J., Huang, Y. M., Bystroff, C., & Collins, C. H. (2013). Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS chemical biology, 8(4), 789-795.
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Revision as of 18:14, 17 October 2021


EsaR D91G mutant

A variant of the Quorum Sensing regulator protein EsaR BBa_K2116001 with the Valine at position 220 substituted by Alanine.

Characterisation

According to the literature[1], the amino acid substitution has increased the sensitivity of this protein to 3OC6HSL molecule. The result of our characterisation is shown below and more details can be found in part BBa_K3989003 .

Figure 1. Fluorescence intensity measurement by plate reader(96-well plate). The measurements were done every one hour and this is the curve of the last test.
Figure 2. Fluorescence intensity measurement by flow cytometry. The samples are taken from the plate, in which the bacteria has been cultured for 7 hours.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1) Shong, J., Huang, Y. M., Bystroff, C., & Collins, C. H. (2013). Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS chemical biology, 8(4), 789-795.