Difference between revisions of "Part:BBa K3776014"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3776014 short</partinfo>
 
<partinfo>BBa_K3776014 short</partinfo>
  
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integrate system
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This Cas7 protein from Vibrio cholerae is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering (see <a href="https://parts.igem.org/Part:BBa_K3776022"> BBa_K3776022</a>). In this system, Cas7 is part of the TniQ-Cascade protein complex which is necessary for RNA-guided DNA integration.(Klompe et al., 2019)
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:07, 17 October 2021


cas7

This Cas7 protein from Vibrio cholerae is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering (see BBa_K3776022). In this system, Cas7 is part of the TniQ-Cascade protein complex which is necessary for RNA-guided DNA integration.(Klompe et al., 2019)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 381
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]