Difference between revisions of "Part:BBa K3733036:Experience"

Latest revision as of 17:59, 17 October 2021

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Applications of BBa_K3733036

We transform E.coli DH5α strain with BBa_K3733036 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.0001mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD₆₀₀ in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).

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Figure 1.The curve of OD₆₀₀ corrected RFU values change with time under induction of different concentrations of thiosulfate

User Reviews

UNIQa8265e5ba7b6fe52-partinfo-00000001-QINU UNIQa8265e5ba7b6fe52-partinfo-00000002-QINU

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 ===Applications of BBa_K3733036===
 <p>
-We transform <i>E.coli</i> DH5α strain with BBa_K3733036 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the reporter. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was spotted into a 96-well plate, and a series of concentration gradients (0mM, 0.0001mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of thiosulfate were added Sodium sulfate solution. Add 2μL of sodium thiosulfate to each well for induction and measure the fluorescence with gain of 75 and OD<sub>600</sub> in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (<b>Figure 1</b>).
+We transform <i>E.coli</i> DH5α strain with BBa_K3733036 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.0001mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD<sub>600</sub> in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (<b>Figure 1</b>).
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