Difference between revisions of "Part:BBa K3733032"

Revision as of 17:42, 17 October 2021

The thiosulfate sensing system under the control of J23103

This part could sense different concentrations of thiosulfate and reflects this difference in fluorescence intensity. Its functionality depends on a two-component system ThsSR, which each constitute a sensor histidine kinase and a response regulator. The ThsS is sensor histidine kinase, which can phosphorylate ThsR in response to thiosulfate. And then the phosphorylated ThsR will binding the site and activate the transcription of P_phsA promoter. In this system, we use J23103 to control the expression of ThsS and ThsR, which in turn controls the differentiation of the system to different thiosulfate concentrations.

The function of BBa_K3733032 is confirmed by iGEM 2021 HZAU-China. See this page:https://parts.igem.org/Part:BBa_K3733032:Experience

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1151
Illegal NheI site found at 1867
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 550
Illegal BamHI site found at 598
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 3065
Illegal AgeI site found at 3188
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1083
Illegal SapI.rc site found at 1909

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3733032 short</partinfo>
-<p>This part could sense different concentrations of thiosulfate and reflects this difference in fluorescence intensity. Its functionality depends on a two-component system ThsSR, which each constitute a sensor histidine kinase and a response regulator. The ThsS is sensor histidine kinase, which can phosphorylate ThsR in response to thiosulfate. And then the phosphorylated ThsR will binding the site and activate the transcription of PphsA promoter. In this system, we use J23103 to control the expression of ThsS and ThsR, which in turn controls the differentiation of the system to different thiosulfate concentrations.</p>
+<p>This part could sense different concentrations of thiosulfate and reflects this difference in fluorescence intensity. Its functionality depends on a two-component system ThsSR, which each constitute a sensor histidine kinase and a response regulator. The ThsS is sensor histidine kinase, which can phosphorylate ThsR in response to thiosulfate. And then the phosphorylated ThsR will binding the site and activate the transcription of P<sub>phsA</sub> promoter. In this system, we use J23103 to control the expression of ThsS and ThsR, which in turn controls the differentiation of the system to different thiosulfate concentrations.</p>
 <p>
 The function of BBa_K3733032 is confirmed by iGEM 2021 HZAU-China. See this page:https://parts.igem.org/Part:BBa_K3733032:Experience