Difference between revisions of "Part:BBa P0140"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_P0140 short</partinfo>
  
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<partinfo>TetR BBa_P0140</partinfo>
 
 
Protein generator converting TIPS to the protein TetR. Used as the input section for [[QPI|Quad Part Inverter]] [[Part:BBa_Q01140]].
 
Protein generator converting TIPS to the protein TetR. Used as the input section for [[QPI|Quad Part Inverter]] [[Part:BBa_Q01140]].
  
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<span class='h3bb'>Sequence and Features
 
<span class='h3bb'>Sequence and Features
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</span>
 
 
<partinfo>BBa_P0140 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_P0140 SequenceAndFeatures</partinfo>
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<partinfo>BBa_P0140 short</partinfo>
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</span>
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<b><h2>Tianjin 2021's characterization</h2></b>
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<partinfo>TetR BBa_P0140</partinfo>
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<b><h2>Tianjin’s 2021 characterization</h2></b>
 
<b><h3>New information of TetR promoter leakage</h3></b>
 
<b><h3>New information of TetR promoter leakage</h3></b>
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<p>Group: Tianjin 2021<br>
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<p >Group: Tianjin 2021
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Author: Ruiqi Liu<br>
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Author: Ruiqi Liu
 
Summary: we added information about TetR promoter leakage.
 
Summary: we added information about TetR promoter leakage.
 
</p>
 
</p>
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[[File:Tianjin-Parts1.png|450px|thumb|right|Figure 1.Design]]
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[[File:Tianjin-Parts2.jpg|300px|thumb|right|Figure 1.Result]]
 
<i><h3>Background</h3></i>
 
<i><h3>Background</h3></i>
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<p>In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.</p>
 
<p>In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.</p>
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<p>Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. </p>
 
<p>Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. </p>
  
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[[File:Tianjin-Parts2.jpg|300px|thumb|left|Figure 2.Result]]
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 +
<h3><i>Design</i></h3>
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[[File:Tianjin-Parts1.png|450px|thumb|left|Figure 2.Design]]
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 16:31, 17 October 2021

PoPS -> TetR [S0163]

Protein generator converting TIPS to the protein TetR. Used as the input section for Quad Part Inverter Part:BBa_Q01140.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

No part name specified with partinfo tag.

Tianjin’s 2021 characterization

New information of TetR promoter leakage

Group: Tianjin 2021 Author: Ruiqi Liu Summary: we added information about TetR promoter leakage.

Figure 1.Result

Background


In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.

Result

Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results.


Design

Figure 2.Design