Difference between revisions of "Part:BBa K1969005"
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This is the full length of CUP1 promoter in yeast Saccharomyces cerevisiae. As the major copper-activated metallothionine in yeast, Cup1p binds and sequesters cuprous copper(I), Cu+, providing the principal method of removing this metal ion from the cell. CUP1 transcription is specifically induced by the copper-dependent transcription activator Cup2p (more commonly known as Ace1p) in response to high levels of copper ions and by Hsf1p in response to heat shock, glucose starvation and oxidation stress. In the presence of copper, Cup1p is also capable of antioxidant activity and thus contributes a significant, albeit minor, role to oxygen radical detoxification, especially in the absence of Cu,Zn-superoxide dismutase Sod1p. | This is the full length of CUP1 promoter in yeast Saccharomyces cerevisiae. As the major copper-activated metallothionine in yeast, Cup1p binds and sequesters cuprous copper(I), Cu+, providing the principal method of removing this metal ion from the cell. CUP1 transcription is specifically induced by the copper-dependent transcription activator Cup2p (more commonly known as Ace1p) in response to high levels of copper ions and by Hsf1p in response to heat shock, glucose starvation and oxidation stress. In the presence of copper, Cup1p is also capable of antioxidant activity and thus contributes a significant, albeit minor, role to oxygen radical detoxification, especially in the absence of Cu,Zn-superoxide dismutase Sod1p. | ||
| + | <h3>Characterization</h3> | ||
| + | [https://2021.igem.org/Team:Toulouse_INSA-UPS]contributed to the characterization of this part by adding a new method for geraniol analysis <br> | ||
| + | (<small>--[[User:Cecilia34|Cecilia34]] 10:10, 23 October 2020 (UTC+2)</small>)<br> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 16:11, 17 October 2021
CUP1 promoter
This is the full length of CUP1 promoter in yeast Saccharomyces cerevisiae. As the major copper-activated metallothionine in yeast, Cup1p binds and sequesters cuprous copper(I), Cu+, providing the principal method of removing this metal ion from the cell. CUP1 transcription is specifically induced by the copper-dependent transcription activator Cup2p (more commonly known as Ace1p) in response to high levels of copper ions and by Hsf1p in response to heat shock, glucose starvation and oxidation stress. In the presence of copper, Cup1p is also capable of antioxidant activity and thus contributes a significant, albeit minor, role to oxygen radical detoxification, especially in the absence of Cu,Zn-superoxide dismutase Sod1p.
Characterization
[1]contributed to the characterization of this part by adding a new method for geraniol analysis
(--Cecilia34 10:10, 23 October 2020 (UTC+2))
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 64
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 64
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 64
- 1000COMPATIBLE WITH RFC[1000]