Difference between revisions of "Part:BBa P0140"
Line 15: Line 15:
 
<b><h2>Tianjin’s 2021 characterization</h2></b>
 
<b><h2>Tianjin’s 2021 characterization</h2></b>
 
<h3>New information of TetR promoter leakage</h3>
 
<h3>New information of TetR promoter leakage</h3>
 +
[[File:Tianjin-Parts2.jpg|300px|thumb|right|Figure 1.Result]]
 
<i><h3>Background</h3></i>
 
<i><h3>Background</h3></i>
  
  
−
[[File:Tianjin-Parts2.jpg|350px|thumb|right|Figure 1.Result]]
+
 
 
<p>In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.</p>
 
<p>In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.</p>
 
<i><h3>Result</h3></i>
 
<i><h3>Result</h3></i>
Line 26: Line 27:
  
 
<h3><i>Design</i></h3>
 
<h3><i>Design</i></h3>
−
[[File:Tianjin-Parts1.png|400px|thumb|left|Figure 2.Design]]
+
[[File:Tianjin-Parts1.png|450px|thumb|left|Figure 2.Design]]
  
  

Revision as of 15:55, 17 October 2021

PoPS -> TetR [S0163]

Protein generator converting TIPS to the protein TetR. Used as the input section for Quad Part Inverter Part:BBa_Q01140.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

TetR BBa_P0140

Tianjin’s 2021 characterization

New information of TetR promoter leakage

Figure 1.Result

Background


In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.

Result

Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results.


Design

Figure 2.Design