Difference between revisions of "Part:BBa P0140"
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<p>Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. </p> | <p>Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. </p> | ||
| − | [[File:Tianjin-Parts2.jpg|350px|thumb||]] | + | [[File:Tianjin-Parts2.jpg|350px|thumb|left|]] |
<h3><i>Design</i></h3> | <h3><i>Design</i></h3> | ||
Revision as of 15:47, 17 October 2021
PoPS -> TetR [S0163]
Protein generator converting TIPS to the protein TetR. Used as the input section for Quad Part Inverter Part:BBa_Q01140.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Tianjin’s 2021 characterization TetR BBa_P0140
New information of TetR promoter leakage
Background
In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.
Result
Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results.
Design