Difference between revisions of "Part:BBa K3733036:Experience"

 
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===Applications of BBa_K3733036===
 
===Applications of BBa_K3733036===
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We transform <i>E.coli</i> DH5α strain with BBa_K3733036 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the reporter. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was spotted into a 96-well plate, and a series of concentration gradients (0mM, 0.0001mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of thiosulfate were added Sodium sulfate solution. Add 2μL of sodium thiosulfate to each well for induction and measure the fluorescence with gain of 75 and OD<sub>600</sub> in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (<b>Figure 1</b>).
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<center><img src="（这里是图片链接）" style="width:793px;height:360px"></center>
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<center><b>Figure 1.</b>The curve of OD<sub>600</sub> corrected RFU values change with time under induction of different concentrations of thiosulfate</center>
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===User Reviews===
 
===User Reviews===

Revision as of 15:14, 17 October 2021


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Please enter how you used this part and how it worked out.

Applications of BBa_K3733036

We transform E.coli DH5α strain with BBa_K3733036 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the reporter. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was spotted into a 96-well plate, and a series of concentration gradients (0mM, 0.0001mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of thiosulfate were added Sodium sulfate solution. Add 2μL of sodium thiosulfate to each well for induction and measure the fluorescence with gain of 75 and OD600 in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).

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Figure 1.The curve of OD600 corrected RFU values change with time under induction of different concentrations of thiosulfate

User Reviews

UNIQ13949cfa378a36ea-partinfo-00000001-QINU UNIQ13949cfa378a36ea-partinfo-00000002-QINU