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Revision as of 14:54, 17 October 2021


panS-loxP-MCS

This shuttle vector was modified based on the shuttle vector BBa_K3228089 designed by iGEM Team Marburg 2019.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
    Illegal BamHI site found at 6
    Illegal XhoI site found at 5663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2378
    Illegal AgeI site found at 4103
  • 1000
    COMPATIBLE WITH RFC[1000]