Difference between revisions of "Part:BBa P0140"

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<p>Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. <p>
 
<p>Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. <p>
 
<img style=”margin-left:10%” width=”80%” src="https://2021.igem.org/wiki/images/c/c4/T--Tianjin--Parts1.png">
 
<img style=”margin-left:10%” width=”80%” src="https://2021.igem.org/wiki/images/c/c4/T--Tianjin--Parts1.png">
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<div class="thumb tright"><div class="thumbinner" style="width:402px;"><a href="/File:Wro_drop.png" class="image"><img alt="" src="/wiki/images/thumb/d/dd/Wro_drop.png/400px-Wro_drop.png" width="400" height="313" class="thumbimage" srcset="/wiki/images/d/dd/Wro_drop.png 1.5x, /wiki/images/d/dd/Wro_drop.png 2x"></a>  <div class="thumbcaption"><div class="magnify"><a href="/File:Wro_drop.png" class="internal" title="Enlarge"></a></div>Figure 1. <i>Drop test analysis of <i>S. cerevisiae</i> EBY.VW4000 transformed with pRS426 plasmid containing YALI0C06424g, YALI08943g and YALI0F19184g hexose transporters from Y. lipolytica under the control of ADH1 (pA) or TEF (pT) promoter.  </i></div></div></div>
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<i><h3>Design</h3></i>
 
<i><h3>Design</h3></i>
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<i><h3>Results</h3></i>
 
<i><h3>Results</h3></i>
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<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_P0140 parameters</partinfo>
 
<partinfo>BBa_P0140 parameters</partinfo>
 
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Revision as of 14:17, 17 October 2021

PoPS -> TetR [S0163]

Protein generator converting TIPS to the protein TetR. Used as the input section for Quad Part Inverter Part:BBa_Q01140.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Tianjin’s 2021 characterization TetR BBa_P0140

New information of TetR promoter leakage

Background

In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.

Result

Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. <p> <img style=”margin-left:10%” width=”80%” src="T--Tianjin--Parts1.png">

<a href="/File:Wro_drop.png" class="image"> 
   <img alt="" srcset="T--Tianjin--Parts1.png" width="400" height="313" class="thumbimage" srcset="/wiki/images/d/dd/Wro_drop.png 1.5x, /wiki/images/d/dd/Wro_drop.png 2x"></a>


Design

<img style=”margin-left:10%” width=”80%” src=" T--Tianjin--Parts2.png" >

Results