Difference between revisions of "Part:BBa P0140"

Revision as of 14:05, 17 October 2021

PoPS -> TetR [S0163]

Protein generator converting TIPS to the protein TetR. Used as the input section for Quad Part Inverter Part:BBa_Q01140.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Tianjin’s 2021 characterization TetR BBa_P0140

New information of TetR promoter leakage
Background

In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.

Result

Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. <p> <img style=”margin-left:10%” width=”80%” src="">

Figure 1. Drop test analysis of <i>S. cerevisiae EBY.VW4000 transformed with pRS426 plasmid containing YALI0C06424g, YALI08943g and YALI0F19184g hexose transporters from Y. lipolytica under the control of ADH1 (pA) or TEF (pT) promoter. </i>

Design

Results

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 Tianjin’s 2021 characterization
 TetR BBa_P0140
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 <b><h2>New information of TetR promoter leakage</h2></b>
 <i><h3>Background</h3></i>
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 <p>Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. <p>
 <img style=”margin-left:10%” width=”80%” src="https://2021.igem.org/wiki/images/c/c4/T--Tianjin--Parts1.png">
+<div class="thumb tright"><div class="thumbinner" style="width:402px;"><a href="/File:Wro_drop.png" class="image"><img alt="" src="/wiki/images/thumb/d/dd/Wro_drop.png/400px-Wro_drop.png" width="400" height="313" class="thumbimage" srcset="/wiki/images/d/dd/Wro_drop.png 1.5x, /wiki/images/d/dd/Wro_drop.png 2x"></a>  <div class="thumbcaption"><div class="magnify"><a href="/File:Wro_drop.png" class="internal" title="Enlarge"></a></div>Figure 1. <i>Drop test analysis of <i>S. cerevisiae</i> EBY.VW4000 transformed with pRS426 plasmid containing YALI0C06424g, YALI08943g and YALI0F19184g hexose transporters from Y. lipolytica under the control of ADH1 (pA) or TEF (pT) promoter.  </i></div></div></div>
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 <i><h3>Design</h3></i>
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 <i><h3>Results</h3></i>
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