Difference between revisions of "Part:BBa K3771015"
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<br><b style="font-size:1.3rem">Description</b>
 
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<br><i>P<sub>lacI</sub>-ompA/oprF</i> is part of the IFN-γ sensing system. lacI promoter regulates the constitutive expression of OmpA/OprF.
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  <br><i>P<sub>lacI</sub>-ompA/oprF</i> is part of the IFN-γ sensing system. <i>lacI</i> promoter regulates the constitutive expression of OmpA/OprF.
 
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<br><b style="font-size:1.3rem">Biology</b>
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<br>When there is no <i>lac</i> repressor, the <i>lacI</i> promoter facilitates the constitutive expression of OmpA/OprF. In the IFN-γ sensing system, binding of IFN-γ to the OmpA/OprF chimeric protein activates the <i>pspA</i> promoter, producing the enzyme required for the synthesis of taurine.
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 11:31, 17 October 2021


PlacI-OmpA/OprF
Description

PlacI-ompA/oprF is part of the IFN-γ sensing system. lacI promoter regulates the constitutive expression of OmpA/OprF.

Biology

When there is no lac repressor, the lacI promoter facilitates the constitutive expression of OmpA/OprF. In the IFN-γ sensing system, binding of IFN-γ to the OmpA/OprF chimeric protein activates the pspA promoter, producing the enzyme required for the synthesis of taurine.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1039
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]