Difference between revisions of "Part:BBa K4090004"
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==Data==
 
==Data==
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[[File:T--SDSZ_China--result_1-2parts.png|700px|thumb|center|Fig.1]]
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According to Fig.1, all the fragments designed have been successfully made, including S1&S2, which are Silicatein combined with INP, and P1&P2, which are S010_pET28b-csgA-linker-mfp5-7His(pET).
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[[File:T--SDSZ China--result 1-4parts.png|700px|thumb|center|Fig.2]]
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Fig.2 represents the results of gel electrophoresis to test the fragment after Gibson Assembly. The length is about 600bp, which, together with the results from sequencing, indicates a positive outcome.
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[[File:T--SDSZ China--result 1-6parts.png|700px|thumb|center|Fig.3]]
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Fig.3 represents qualitative OD values, showing that there is sufficient amount of silicatein produced, since:
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•Both NC Control are darker (NC1 remain as yellow which indicates that it needs MORE NaOH to turn blue, it contains more acids).
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•Both silicatein mixture (with / without homogenization) have higher OD810nm, meaning that there are more bacteria inside the test tube (the solution is thicker / not as clear as NCs).
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[[File:T--SDSZ China--result 1-7parts.jpeg|700px|thumb|center|Fig.4]]
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Fig.4 shows the results from TEM testing. Dark proportions represent silicon dioxide produced on the surface of cells.
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[[File:T--SDSZ China--result 1-3parts.png|700px|thumb|center|Fig.5]]
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Revision as of 11:04, 17 October 2021


Silicatein

Silicatein is a kind of mineralized protein that helps to form silicon dioxide

Data

Fig.1

According to Fig.1, all the fragments designed have been successfully made, including S1&S2, which are Silicatein combined with INP, and P1&P2, which are S010_pET28b-csgA-linker-mfp5-7His(pET).

Fig.2

Fig.2 represents the results of gel electrophoresis to test the fragment after Gibson Assembly. The length is about 600bp, which, together with the results from sequencing, indicates a positive outcome.

Fig.3

Fig.3 represents qualitative OD values, showing that there is sufficient amount of silicatein produced, since: •Both NC Control are darker (NC1 remain as yellow which indicates that it needs MORE NaOH to turn blue, it contains more acids). •Both silicatein mixture (with / without homogenization) have higher OD810nm, meaning that there are more bacteria inside the test tube (the solution is thicker / not as clear as NCs).

Fig.4

Fig.4 shows the results from TEM testing. Dark proportions represent silicon dioxide produced on the surface of cells.

Fig.5


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 664
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Hale, L. V., Ma, Y. F. & Santerre, R. F. Semi-quantitative fuorescence analysis of calcein binding as a measurement of in vitro mineralization. Calcif. Tissue Int. 67, 80-84(2000). Ramachandran S K.
[2] Dick J, Windt W D, Graef B D, et al. Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species[J]. Biodegradation, 2016(4): 357-367.
[3] Qian C X, Chen H C, Ren L F, et al. Self-healing of early age cracks in cement-based materials by mineralization of carbonic anhydrase microorganism[J]. Frontiers in Microbiology, 2015(6): 1-9.