Difference between revisions of "Part:BBa K4090000"
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[[File:T--SDSZ China--result 1-4parts.png|700px|thumb|center|Fig.1-2]]
 
[[File:T--SDSZ China--result 1-4parts.png|700px|thumb|center|Fig.1-2]]
 
Fig.1-2 represents the results of gel electrophoresis to test the fragment after Gibson Assembly. The length is about 600bp, which, together with the results from sequencing, indicates a positive outcome.
 
Fig.1-2 represents the results of gel electrophoresis to test the fragment after Gibson Assembly. The length is about 600bp, which, together with the results from sequencing, indicates a positive outcome.
−
[[File:T--SDSZ_China--result_1-6.png|700px|thumb|center|Fig.1-3]]
+
[[File:T--SDSZ China--result 1-6parts.png|700px|thumb|center|Fig.1-3]]
 
Fig.1-3 represents qualitative OD values, showing that there is sufficient amount of silicatein produced, since:  
 
Fig.1-3 represents qualitative OD values, showing that there is sufficient amount of silicatein produced, since:  
 
•Both NC Control are darker (NC1 remain as yellow which indicates that it needs MORE NaOH to turn blue, it contains more acids).
 
•Both NC Control are darker (NC1 remain as yellow which indicates that it needs MORE NaOH to turn blue, it contains more acids).
 
•Both silicatein mixture (with / without homogenization) have higher OD810nm, meaning that there are more bacteria inside the test tube (the solution is thicker / not as clear as NCs).
 
•Both silicatein mixture (with / without homogenization) have higher OD810nm, meaning that there are more bacteria inside the test tube (the solution is thicker / not as clear as NCs).
−
[[File:T--SDSZ_China--result_1-7.png|700px|thumb|center|Fig.1-4]]
+
[[File:T--SDSZ China--result 1-7.png|700px|thumb|center|Fig.1-4]]
 
Fig.1-4 shows the results from TEM testing. Dark proportions represent silicon dioxide produced on the surface of cells.
 
Fig.1-4 shows the results from TEM testing. Dark proportions represent silicon dioxide produced on the surface of cells.
 
[[File:T--SDSZ_China--result_1-3-2.png|700px|thumb|center|Fig.1-5]]
 
[[File:T--SDSZ_China--result_1-3-2.png|700px|thumb|center|Fig.1-5]]

Revision as of 09:48, 17 October 2021

Ice Nucleation Protein(INP)

ice nucleation protein(INP) is a kind of transmembrane protein, which can help proteins to play their role outside the membrane.

Data

Fig.1-1

According to Fig.1-1, all the fragments designed have been successfully made, including S1&S2, which are Silicatein combined with INP, and P1&P2, which are S010_pET28b-csgA-linker-mfp5-7His(pET).

Fig.1-2

Fig.1-2 represents the results of gel electrophoresis to test the fragment after Gibson Assembly. The length is about 600bp, which, together with the results from sequencing, indicates a positive outcome.

Fig.1-3

Fig.1-3 represents qualitative OD values, showing that there is sufficient amount of silicatein produced, since: •Both NC Control are darker (NC1 remain as yellow which indicates that it needs MORE NaOH to turn blue, it contains more acids). •Both silicatein mixture (with / without homogenization) have higher OD810nm, meaning that there are more bacteria inside the test tube (the solution is thicker / not as clear as NCs).

File:T--SDSZ China--result 1-7.png
Fig.1-4

Fig.1-4 shows the results from TEM testing. Dark proportions represent silicon dioxide produced on the surface of cells.

File:T--SDSZ China--result 1-3-2.png
Fig.1-5



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 321
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 396
    Illegal AgeI site found at 498
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 229

References

[1]ZHU T, PAULO C, MERROUN, M L, et al. Potential application of biomineralization by Synechococcus PCC8806 for concrete restoration[J]. Sedimentology, 2013,61(1):1-21 [2]Lechner, Carolin C., and Christian FW Becker. "A sequence‐function analysis of the silica precipitating silaffin R5 peptide." Journal of Peptide Science 20.2 (2014): 152-158. [3]Poulsen, Nicole, and Nils Kroger. "Silica morphogenesis by alternative processing of silaffins in the diatom Thalassiosira pseudonana." Journal of Biological Chemistry 279.41 (2004): 42993-42999.