Difference between revisions of "Part:BBa K3771013"
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<partinfo>BBa_K3771013 short</partinfo> | <partinfo>BBa_K3771013 short</partinfo> | ||
− | to | + | <br><b style="font-size:1.3rem">Description</b> |
+ | <br> | ||
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+ | <br><i>P<sub>lacI</sub></i>-OFP is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.<br> | ||
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+ | <br><b style="font-size:1.3rem">Biology</b> | ||
+ | <br> | ||
+ | |||
+ | <br>Without the presence of the LacI repressor, the LacI promoter facilitates the constitutive expression of OFP.<br> | ||
+ | |||
+ | <br><b style="font-size:1.3rem">Characterization</b> | ||
+ | <br> | ||
+ | |||
+ | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
+ | <img src="https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;"> | ||
+ | </div> | ||
+ | <p align="center">Double digestion results are shown in Fig. 2.</p> | ||
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+ | |||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 09:33, 17 October 2021
PlacI-OFP
Description
PlacI-OFP is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.
Biology
Without the presence of the LacI repressor, the LacI promoter facilitates the constitutive expression of OFP.
Characterization
<img src="" style="width:35%;">
Double digestion results are shown in Fig. 2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]