Difference between revisions of "Part:BBa K3815006"

Revision as of 09:02, 17 October 2021

No part name specified with partinfo tag. This part is used His tag to purify the peptides. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein.In this part, the purpose protein is CecropinA derived from a moth. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. This sequence got from the moth. In our experiment, we used it to kill the bacteria in the vase water.

Sequence and Features No part name specified with partinfo tag.

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 <partinfo>BBa_K3815035 short</partinfo>
+This part is used His tag to purify the peptides. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein.In this part, the purpose protein is CecropinA derived from a moth. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. This sequence got from the moth. In our experiment, we used it to kill the bacteria in the vase water.
-The purified His-tagged peptide was recovered by Ni chromatography, and DTT was added to cleave the N-terminus of the intein.The purpose peptide is CecropinA. This is an antimicrbial peptide that  that can inhibit the both gram-negative bacteria and gram-positive bacteria. In our experiment, we can use it to inhibit the bacteria in the vase water.
 <!-- Add more about the biology of this part here