Difference between revisions of "Part:BBa K3771047"
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| − | <br><i>P<sub>katG</sub></i> is the promoter of the <i>katG</i> gene. KatG protein has the function of bifunctional hydroperoxidase I (HPI) and plays an important role in oxidative stress response. The <i>katG</i> promoter (P<sub>katG</sub>) is stimulated by oxidative stress, leading to the expression of the downstream gene. Besides, P<sub>katG</sub> is activated by OxyR protein<sup>[1]</sup>. | + | <br><i>P<sub>katG</sub></i> is the promoter of the <i>katG</i> gene. KatG protein has the function of bifunctional hydroperoxidase I (HPI) and plays an important role in oxidative stress response. The <i>katG</i> promoter (<i>P<sub>katG</sub></i>) is stimulated by oxidative stress, leading to the expression of the downstream gene. Besides, <i>P<sub>katG</sub></i> is activated by OxyR protein<sup>[1]</sup>. |
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<img src="https://static.igem.org/mediawiki/parts/1/14/T--NCKU_Tainan--katG_genome.png" style="width:35%;"> | <img src="https://static.igem.org/mediawiki/parts/1/14/T--NCKU_Tainan--katG_genome.png" style="width:35%;"> | ||
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| − | <p align="center">Figure 1. Transcription unit of the <i>katG</i> gene in E. coli K-12<sup>[2]</sup>.</p></html> | + | <p align="center">Figure 1. Transcription unit of the <i>katG</i> gene in <i>E. coli</i> K-12<sup>[2]</sup>.</p></html> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <br><b style="font-size:1.3rem">Usage and Biology</b> | ||
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| + | <br>After conducting colony PCR from the <i>E. coli</i> MG1655, the <i>P<sub>katG</sub></i> fragment can be amplified from the chromosome and the experiment result can be checked by agarose gel electrophoresis. The result is shown in Figure 2. | ||
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| + | <html><div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
| + | <img src="https://2021.igem.org/wiki/images/d/d3/T--NCKU_Tainan--PkatG_PCR.png" style="width:35%;"> | ||
| + | </div> | ||
| + | <p align="center">Figure 2. The electrophoresis result of <i>P<sub>katG</sub></i> fragment from the chromosome PCR. M: Marker; Lane 1: katG promoter (<i>P<sub>katG</sub></i>) (217 bp). | ||
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Revision as of 08:41, 17 October 2021
PkatG
Description
PkatG is the promoter of the katG gene. KatG protein has the function of bifunctional hydroperoxidase I (HPI) and plays an important role in oxidative stress response. The katG promoter (PkatG) is stimulated by oxidative stress, leading to the expression of the downstream gene. Besides, PkatG is activated by OxyR protein[1].
Figure 1. Transcription unit of the katG gene in E. coli K-12[2].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]