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| − | <br><b style="font-size:1.3rem">Achievements</b>
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| − | <br>Confirmation of CSAD enzyme expression under oxidative stress by western blot.<br>
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| − | <br><b style="font-size:1.3rem">Process</b>
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| − | <br>To confirm that the CSAD enzyme is successfully expressed when induced by paraquat, the western blot was performed. When the OD600 of the bacterial culture reached 0.5, we added 0.1 mM paraquat into the culture and collected samples at 2, 4, 6 hours after paraquat was added. The result confirmed that CSAD was expressed under oxidative stress. The sample collected at 6 hours after paraquat was added had the greatest expression, and CSAD expression increases as the time allowed for paraquat to induce the SoxS promoter increases. (Figure #).<br>
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| − | <img src="https://2021.igem.org/wiki/images/5/58/T--NCKU_Tainan--soxR-CSAD_WB.png" style="width:35%;"></html>
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| − | <p align="center">Figure #. The western blot result. CSAD (~55 kDa); –: control; PQ: 0.1 mM paraquat.</p>
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