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<br><br><b style="font-size:1.3rem">Description
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<br><br>Outer membrane protein A (OmpA) is a 35 kDa transmembrane protein found in many enterobacteria. It is responsible for maintaining the stability of the bacterial membrane and cell signaling. [1]
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<br><br><b style="font-size:1.3rem">Biology
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<br><br>In E. coli, the β-barrel conformation of OmpA is composed of extracellular loops that help play a role in the detection of outer membrane stress and binding of extracellular molecules. OmpA also serves as a porin channel that regulates water transport within the bacterial cell. [2]
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<br><br><b style="font-size:1.3rem">Usage
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<br><br>In our project, the sequence of OmpA protein was used to construct the OmpA/OprF chimeric protein.
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<img src="https://2021.igem.org/File:T--NCKU_Tainan--ompA_oprF.gif" style="width:40%;">
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Revision as of 07:37, 17 October 2021


Outer Membrane Protein A (OmpA)



Description

Outer membrane protein A (OmpA) is a 35 kDa transmembrane protein found in many enterobacteria. It is responsible for maintaining the stability of the bacterial membrane and cell signaling. [1]

Biology

In E. coli, the β-barrel conformation of OmpA is composed of extracellular loops that help play a role in the detection of outer membrane stress and binding of extracellular molecules. OmpA also serves as a porin channel that regulates water transport within the bacterial cell. [2]

Usage

In our project, the sequence of OmpA protein was used to construct the OmpA/OprF chimeric protein.











Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 881
    Illegal BamHI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]