Difference between revisions of "Part:BBa K3784000"

Revision as of 07:14, 17 October 2021

acdA (encoding 3-(aryl) acrylate reductase)

acdA is from the FLD gene cluster of Clostridium sporogenes, involved in the conversion of tryptophan to 3-Indolepropionic acid. In the five-step metabolic pathway we reconstructed, acdA encodes 3-(aryl)acrylate reductase, which catalyze indole acrylic acid to produce indole propionic acid.

Characterization

We linked acdA fragment and pET-28a plasmid, then transferred the recombinant plasmid into E. coli and test it by colony PCR.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 291
Illegal SpeI site found at 619
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 291
Illegal SpeI site found at 619
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1141
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 291
Illegal SpeI site found at 619
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 291
Illegal SpeI site found at 619
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3784000 short</partinfo>
-acdA is from the FLD gene cluster of Clostridium sporogenes, involved in the conversion of tryptophan to 3-Indolepropionic acid. In the five-step metabolic pathway we reconstructed, acdA encodes 3-(aryl) acylate, which catalyze indole acrylic acid to produce indole propionic acid.
+acdA is from the FLD gene cluster of Clostridium sporogenes, involved in the conversion of tryptophan to 3-Indolepropionic acid. In the five-step metabolic pathway we reconstructed, acdA encodes 3-(aryl)acrylate reductase, which catalyze indole acrylic acid to produce indole propionic acid.
 ===Characterization===