Difference between revisions of "Part:BBa K3771018"
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<partinfo>BBa_K3771018 short</partinfo> | <partinfo>BBa_K3771018 short</partinfo> | ||
− | to | + | <br><b style="font-size:1.3rem">Description</b> |
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+ | <br>This composite part is part of a plasmid used in the IFN-γ induction assay to test the function of the IFN-γ sensing system. It consists of the ompA/oprF gene regulated by the ompA promoter (PompA-ompA/oprF) and the orange fluorescent protein (OFP) gene regulated by the pspA promoter (PpspA-ofp).<br> | ||
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+ | <br><b style="font-size:1.3rem">Biology</b> | ||
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+ | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
+ | <img src="https://2021.igem.org/wiki/images/a/af/T--NCKU_Tainan--ompA_oprF.gif" style="width:35%;"> | ||
+ | </div> | ||
+ | <p align="center">Figure. 1 Structure of OmpA/OprF chimeric protein</p> | ||
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Revision as of 06:39, 17 October 2021
PpspA-OFP-PompA-OmpA/OprF
Description
This composite part is part of a plasmid used in the IFN-γ induction assay to test the function of the IFN-γ sensing system. It consists of the ompA/oprF gene regulated by the ompA promoter (PompA-ompA/oprF) and the orange fluorescent protein (OFP) gene regulated by the pspA promoter (PpspA-ofp).
Biology
<img src="" style="width:35%;">
Figure. 1 Structure of OmpA/OprF chimeric protein
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 12
Illegal BamHI site found at 2158 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]