Difference between revisions of "Part:BBa K3740004"
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<partinfo>BBa_K3740004 short</partinfo> | <partinfo>BBa_K3740004 short</partinfo> | ||
===Description=== | ===Description=== | ||
− | Involves in the translocation across the outer membrane,we used it to construct chimeric bacteriocin SE. | + | Involves in the translocation across the outer membrane, we used it to construct chimeric bacteriocin SE. |
===Sequence and Features=== | ===Sequence and Features=== | ||
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=2021 SZPT-China= | =2021 SZPT-China= | ||
<h3>Biology</h3> | <h3>Biology</h3> | ||
− | <p>Bacteriocin activity proteins have three distinct domains arranged from N to C terminus that include receptor,translocase,and nuclease (with immunity protein-binding)domains. The translocation domain are species specific.</p> | + | <p>Bacteriocin activity proteins have three distinct domains arranged from N to C terminus that include receptor, translocase, and nuclease (with immunity protein-binding)domains. The translocation domain are species specific.</p> |
<h3>References</h3> | <h3>References</h3> | ||
<p>[1] Gupta S, Bram E E, Weiss R. Genetically programmable pathogen sense and destroy. [J]. Acs Synthetic Biology, 2013, 2(12):715-723.</p> | <p>[1] Gupta S, Bram E E, Weiss R. Genetically programmable pathogen sense and destroy. [J]. Acs Synthetic Biology, 2013, 2(12):715-723.</p> | ||
<p>[2] Michel-Briand Y, Baysse C. The pyocins of Pseudomonas aeruginosa. [J]. Biochimie, 2002, 84(5-6):499-510</p> | <p>[2] Michel-Briand Y, Baysse C. The pyocins of Pseudomonas aeruginosa. [J]. Biochimie, 2002, 84(5-6):499-510</p> |
Revision as of 04:00, 17 October 2021
Translocationl domain of Pyocin S2
Description
Involves in the translocation across the outer membrane, we used it to construct chimeric bacteriocin SE.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 732
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 531
Illegal AgeI site found at 621 - 1000COMPATIBLE WITH RFC[1000]
2021 SZPT-China
Biology
Bacteriocin activity proteins have three distinct domains arranged from N to C terminus that include receptor, translocase, and nuclease (with immunity protein-binding)domains. The translocation domain are species specific.
References
[1] Gupta S, Bram E E, Weiss R. Genetically programmable pathogen sense and destroy. [J]. Acs Synthetic Biology, 2013, 2(12):715-723.
[2] Michel-Briand Y, Baysse C. The pyocins of Pseudomonas aeruginosa. [J]. Biochimie, 2002, 84(5-6):499-510