Difference between revisions of "Part:BBa K3888011"
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The part basically consists of two main gene: HoxG1 and HoxG1, which encode the large subunit and small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase respectively.
 
The part basically consists of two main gene: HoxG1 and HoxG1, which encode the large subunit and small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase respectively.
 
Other key features also include a natural (wild) type of RBS, B0032m, and a pTac promoter which is controlled by LacI(induced by IPTG) and lac operator .  
 
Other key features also include a natural (wild) type of RBS, B0032m, and a pTac promoter which is controlled by LacI(induced by IPTG) and lac operator .  
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[[File:T--SJTang--BBa_K3888011-1.png]]
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3888011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3888011 SequenceAndFeatures</partinfo>
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[[File:T--SJTang--BBa_K3888011-1.png]]
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<!-- Uncomment this to enable Functional Parameter display  
 
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Revision as of 03:53, 17 October 2021


[Ni-Fe] Hydrogenase Core gene HoxG1 and HoxK1

This composite part is used for heterologous expression of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] Hydrogenase is extremely sensitive to oxygen. By expressing oxygen-tolerant hydrogenase in E.coli, the oxygen tolerance will increase thus increases the amount of hydrogen produced by hydrogenase in E.coli. The strain of E.coli we used in the experiments is E.coli BL21. The part basically consists of two main gene: HoxG1 and HoxG1, which encode the large subunit and small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase respectively. Other key features also include a natural (wild) type of RBS, B0032m, and a pTac promoter which is controlled by LacI(induced by IPTG) and lac operator .

T--SJTang--BBa K3888011-1.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 363
    Illegal XbaI site found at 465
    Illegal SpeI site found at 531
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 531
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 678
    Illegal BamHI site found at 1593
    Illegal BamHI site found at 2932
    Illegal XhoI site found at 1915
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 363
    Illegal XbaI site found at 465
    Illegal SpeI site found at 531
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 363
    Illegal XbaI site found at 465
    Illegal SpeI site found at 531
    Illegal AgeI site found at 1809
  • 1000
    COMPATIBLE WITH RFC[1000]