Difference between revisions of "Part:BBa K3389003"

 
Line 4: Line 4:
  
 
This part codes for an expression system for the RAD27 flap endonuclease 1 ("flappase") from Saccharomyces cerevisiae, including a 10x His tag for purification purposes. RAD27, also known as FEN-1, is a structure-specific flap endonuclease that will cleave overlapping oligonucleotides at the site of a single target nucleotide in the context of a known sequence. One use for this is to distinguish between single nucleotide polymorphisms. The expression system has a T7 promoter, a strong RBS, and a 10x His tag.
 
This part codes for an expression system for the RAD27 flap endonuclease 1 ("flappase") from Saccharomyces cerevisiae, including a 10x His tag for purification purposes. RAD27, also known as FEN-1, is a structure-specific flap endonuclease that will cleave overlapping oligonucleotides at the site of a single target nucleotide in the context of a known sequence. One use for this is to distinguish between single nucleotide polymorphisms. The expression system has a T7 promoter, a strong RBS, and a 10x His tag.
 +
 +
 +
===Characterization===
 +
Add more about this part here
 +
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:01, 17 October 2021


RAD27 Flappase expression construct with 10x His tag

This part codes for an expression system for the RAD27 flap endonuclease 1 ("flappase") from Saccharomyces cerevisiae, including a 10x His tag for purification purposes. RAD27, also known as FEN-1, is a structure-specific flap endonuclease that will cleave overlapping oligonucleotides at the site of a single target nucleotide in the context of a known sequence. One use for this is to distinguish between single nucleotide polymorphisms. The expression system has a T7 promoter, a strong RBS, and a 10x His tag.


Characterization

Add more about this part here


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 311
    Illegal BglII site found at 704
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216