Difference between revisions of "Part:BBa K3766023"

 
 
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<partinfo>BBa_K3766022 short</partinfo>
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AmpR promoter + RBS (with one mutation)
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This part is the promoter and RBS of the ampicillin resistance gene, AmpR. It contains one mutation, G59T that was introduced fortuitously during PCR amplification. This mutation does not impair the expression of AmpR as deduced by the fact that the capacity of ''E. coli'' cells to grow in the presence of ampicillin is not disrupted.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3766022 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3766023 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3766022 parameters</partinfo>
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<partinfo>BBa_K3766023 parameters</partinfo>
 
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Latest revision as of 23:19, 16 October 2021


AmpR promoter + RBS (with one mutation)

This part is the promoter and RBS of the ampicillin resistance gene, AmpR. It contains one mutation, G59T that was introduced fortuitously during PCR amplification. This mutation does not impair the expression of AmpR as deduced by the fact that the capacity of E. coli cells to grow in the presence of ampicillin is not disrupted.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]