Difference between revisions of "Part:BBa K3766013"

Latest revision as of 22:26, 16 October 2021

mCherry (reverse orientation)

This codon optimized mCherry for Escherichia coli K12 is the BBa_K2448004 in reverse orientation.

Usage and Biology

mCherry [1] is a fluorescent monomeric protein widely used in biotechnology. Like the majority of red fluorescent proteins, it is derived from the Red Fluorescent protein from Discosoma sp. mCherry is sometimes preferred over other fluorophores due to its color, its rapid maturation time (t0.5=15 min), its lower brightness (around 50% of eGFP) as well as its improved photostability and resistance to bleaching.

References

[1] Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology (2004) 22: 1567–1572.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 25
Illegal AgeI site found at 137
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3766013 short</partinfo>
-mCherry (reverse orientation)
+This codon optimized mCherry for ''Escherichia coli'' K12 is the [[Part:BBa_K2448004|BBa_K2448004]] in reverse orientation.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+mCherry [1] is a fluorescent monomeric protein widely used in biotechnology. Like the majority of red fluorescent proteins, it is derived from the Red Fluorescent protein from ''Discosoma'' sp. mCherry is sometimes preferred over other fluorophores due to its color, its rapid maturation time (t0.5=15 min), its lower brightness (around 50% of eGFP) as well as its improved photostability and resistance to bleaching.
+===References===
+[1] Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from ''Discosoma'' sp. red fluorescent protein. Nature Biotechnology (2004) 22: 1567–1572.
 <!-- -->