Difference between revisions of "Part:BBa K3989012"

 
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<partinfo>BBa_K3989012 short</partinfo>
 
<partinfo>BBa_K3989012 short</partinfo>
  
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Transmembrane protein ClyA with two epitopes linked
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EsaR is a regulator protein which regulates the promoter of a bacterial Quorum Sensing system. By interacting with the Quorum Sensing molecules(a class of molecules called AHL.In our project, the molecule is called 3OC6HSL since it shows a higher sensitivity), it can either act as a repressor or an activator depending on the location of the promoter it binds.
  
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<!-- Add more about the biology of this part here
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===How it works===
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===Usage and Biology===
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In the P<sub>esa</sub> promoter controlled Quorum Sensing system, EsaR acts as a regulator that can bind to a specific site of the promoter P<sub>esa</sub>. Depending on the different types of the P<sub>esa</sub>(P<sub>esaR</sub> and P<sub>esaS</sub>), EsaR can be either a repressor(for P<sub>esaR</sub>) or an activator(for P<sub>esaR</sub>). The detailed mechanism is shown below:
  
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<figure>
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  <img src="https://static.igem.org/mediawiki/parts/3/37/21_UZurich_EsaR_activation_repression.jpeg">
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  <figcaption><b>Figure 1</b>. EsaR acts as either an activator or a repressor.</figcaption>
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</figure>
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From the figure we can see that, the EsaR protein binds to a specific site called the <it>esa box</it>. This binding site has a different location on P<sub>esaR</sub> and <sub>esaS</sub> and the EsaR binds to it when there is no AHL
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molecules exist. However, when interacting with AHL, the EsaR will perform an allocation change. Subsequently, it will dislocate from the binding site and will either recruits or interferes the RNA polymerase for downstream gene expression.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 20:38, 16 October 2021


EsaR regulator

EsaR is a regulator protein which regulates the promoter of a bacterial Quorum Sensing system. By interacting with the Quorum Sensing molecules(a class of molecules called AHL.In our project, the molecule is called 3OC6HSL since it shows a higher sensitivity), it can either act as a repressor or an activator depending on the location of the promoter it binds.

How it works

In the Pesa promoter controlled Quorum Sensing system, EsaR acts as a regulator that can bind to a specific site of the promoter Pesa. Depending on the different types of the Pesa(PesaR and PesaS), EsaR can be either a repressor(for PesaR) or an activator(for PesaR). The detailed mechanism is shown below:

<figure> 

 <img src="21_UZurich_EsaR_activation_repression.jpeg">
 <figcaption>Figure 1. EsaR acts as either an activator or a repressor.</figcaption>

</figure>

From the figure we can see that, the EsaR protein binds to a specific site called the <it>esa box</it>. This binding site has a different location on PesaR and esaS and the EsaR binds to it when there is no AHL molecules exist. However, when interacting with AHL, the EsaR will perform an allocation change. Subsequently, it will dislocate from the binding site and will either recruits or interferes the RNA polymerase for downstream gene expression. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]