Difference between revisions of "Part:BBa J70301:Design"
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GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg | GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg | ||
| + | |||
| + | |||
| + | 474647 - 474537 (reverse orientation) from NC_006055 | ||
===Source=== | ===Source=== | ||
Revision as of 21:38, 29 March 2009
Heat shock inducible promoter for Me. florum
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Low gc content, difficult PCR primers
primers:
GTTTCTTC GAATTC GCGGCCGC T TCTAGA G caaataacggataattataaaataaaac
GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg
474647 - 474537 (reverse orientation) from NC_006055
Source
PCR from lon protease promoter of NC_006055.