Difference between revisions of "Part:BBa J70301:Design"

(Design Notes)
(Design Notes)
Line 11: Line 11:
 
primers:
 
primers:
 
caaataacggataattataaaataaaac
 
caaataacggataattataaaataaaac
 +
ctatgtggttaaatatatattatcatattg
  
 
===Source===
 
===Source===

Revision as of 21:16, 29 March 2009

Heat shock inducible promoter for Me. florum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Low gc content, difficult PCR primers


primers: caaataacggataattataaaataaaac ctatgtggttaaatatatattatcatattg

Source

PCR from lon protease promoter of NC_006055.

References