Difference between revisions of "Part:BBa J70300:Design"
(→Design Notes) |
(→Design Notes) |
||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | XbaI site removed from the upstream region, by t->c mutation at 16. | + | XbaI site removed from the upstream region, by t->c mutation at 16 inserted into the F primer. |
Revision as of 02:34, 29 March 2009
Strong Me. florum promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
XbaI site removed from the upstream region, by t->c mutation at 16 inserted into the F primer.
GTTTCTTC GAATTC GCGGCCGC T TCTAGA G cagttaaaaacattccagataagtttgc
GTTTCTTC CTGCAG CGGCCGC T ACTAGT A attaaattcaaaatatcttctgcttc
Source
53556 53678 approximately from NC_006055