Difference between revisions of "Part:BBa K3735008"

Revision as of 17:12, 16 October 2021

Coding sequence

In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with high intensity. (BBa_J23119). Our model group also modeling the expression of ssuE, and found that ssuE with middle and high promoters seriously affects the respiratory metabolism of bacteria. Then we ruled out promoter of high and middle intensity, which means, low intensity promoter is suitable for gene ssuE. With promoter and RBS, the composite part can be directly imported into plasmid backbone and express ssuE gene. If you want to learn about the detailed introduction of ssuE, you can click the link BBa_K3735002.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1537
Illegal BglII site found at 2854
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 329
Illegal NgoMIV site found at 644
Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 1151
Illegal NgoMIV site found at 1574
Illegal NgoMIV site found at 2675
Illegal NgoMIV site found at 2924
Illegal AgeI site found at 671
Illegal AgeI site found at 1217
Illegal AgeI site found at 1373
Illegal AgeI site found at 1514
Illegal AgeI site found at 1937
Illegal AgeI site found at 2294
Illegal AgeI site found at 2465
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3735008 short</partinfo>
-aaaa
+In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with high intensity. (BBa_J23119).
+Our model group also modeling the expression of ssuE, and found that ssuE with middle and high promoters seriously affects the respiratory metabolism of bacteria. Then we ruled out promoter of high and middle intensity, which means, low intensity promoter is suitable for gene ssuE. With promoter and RBS, the composite part can be directly imported into plasmid backbone and express ssuE gene. If you want to learn about the detailed introduction of ssuE, you can click the link BBa_K3735002.