Difference between revisions of "Part:BBa K3893028"
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[[File:T--Ecuador--K3893028_sbolv.png|500px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the device ]]
 
[[File:T--Ecuador--K3893028_sbolv.png|500px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the device ]]
  
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This devise was used to characterize the pLux promoter  
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This device was used to characterize the pLux promoter. It is an AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.
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AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.
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This composite part, together with [https://parts.igem.org/Part:BBa_K3893027 K3893027], is a component of the intermediate part [https://parts.igem.org/Part:BBa_K3893029 K3893029]  to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030 K3893030]) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.
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This composite part, together with [https://parts.igem.org/Part:BBa_K3893029 K3893029], is an intermediate composite part to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030 K3893030]) of our Agrobactory 593. It includes a TU that expresses GFP under rhe control of the pLux promoter and a TU that expresses LuxR constitutively. It is the first half of our population oscillator release device.
  
 
This transcriptional unit is composed of the following standardized composite parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:  
 
This transcriptional unit is composed of the following standardized composite parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:  

Revision as of 16:25, 16 October 2021

AHL induced expression of GFP (including luxR constitutive expression)

domestication.
Figure 1. SBOL Visual representation of the device

This device was used to characterize the pLux promoter. It is an AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.


This composite part, together with K3893029, is an intermediate composite part to assemble the release system device (K3893030) of our Agrobactory 593. It includes a TU that expresses GFP under rhe control of the pLux promoter and a TU that expresses LuxR constitutively. It is the first half of our population oscillator release device.

This transcriptional unit is composed of the following standardized composite parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

  • BBa_K2656122: a Level 1 transcriptional unit expresing GFP under the control of the pux promoter (Golden Braid compatible)
  • BBa_K2656114: a Level 1 transcriptional unit constitutively expressing luxR gene (Golden Braid compatible)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 956
    Illegal NheI site found at 979
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]