Difference between revisions of "Part:BBa K3753015:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
 
The UAS<sub>CLB</sub> and the promoter of <em>tef2</em> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The green fluorescent protein and the terminator CYC1(GFP-CYC1) was obtained by PCR amplification using pRS415-GFP-CYC1 plasmid as template.
 
The UAS<sub>CLB</sub> and the promoter of <em>tef2</em> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The green fluorescent protein and the terminator CYC1(GFP-CYC1) was obtained by PCR amplification using pRS415-GFP-CYC1 plasmid as template.
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===Source===
 
===Source===

Latest revision as of 15:59, 16 October 2021


UASCLB-pTEF-GFP-CYC1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 747
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 747
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 747
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 747
    Illegal NgoMIV site found at 1269
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2423


Design Notes

The UASCLB and the promoter of tef2 was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The green fluorescent protein and the terminator CYC1(GFP-CYC1) was obtained by PCR amplification using pRS415-GFP-CYC1 plasmid as template.

Source

Saccharomyces cerevisiae

References