Difference between revisions of "Part:BBa K3989001:Design"

 
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===Source===
 
===Source===
  
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Sanchez, M. I., & Ting, A. Y. (2020). Directed evolution improves the catalytic efficiency of TEV protease. Nature methods, 17(2), 167-174.
+
Original TEV protease sequence and structure. PDB: 1LVM
  
 
===References===
 
===References===
 +
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1) Denard, C. A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., ... & Iverson, B. L. (2021). YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants. ACS Synthetic Biology, 10(1), 63-71.
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</HTML>

Revision as of 15:59, 16 October 2021


uTEV3, efficiency-enhanced TEV protease variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sanchez, M. I., & Ting, A. Y. (2020). Directed evolution improves the catalytic efficiency of TEV protease. Nature methods, 17(2), 167-174.


Source

Original TEV protease sequence and structure. PDB: 1LVM

References

1) Denard, C. A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., ... & Iverson, B. L. (2021). YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants. ACS Synthetic Biology, 10(1), 63-71.