Difference between revisions of "Part:BBa K3753012"

Revision as of 15:59, 16 October 2021

UAS_CIT-pTEF

UAS_CIT-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from the mitochondrial citrate synthase gene cit2, and the core promoter tef2. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels.

Characterization

Fig.1 The fluorescence intensity of GFP expressed by engineered promoters

When assembled with GFP BBa_K3112009, and BBa_K3384311 in pRS426, this construct expressed GFP. Then the activity of the bybrid promoter can be quantitatively measured through flow cytometer. As is shown in Fig. 1, the UAS_TEF-pTEF promoter strength was expanded beyond the pTEF by 1.2-fold.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 845
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 845
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 845
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 845
Illegal NgoMIV site found at 1367
1000
COMPATIBLE WITH RFC[1000]

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 [[File:Gold.png|width='100%' valign='top'| |center|thumb|480*500px|''<b>Fig.1</b> The fluorescence intensity of GFP expressed by engineered promoters]]
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-When assembled with GFP <partinfo>BBa_K3112009</partinfo>, and <partinfo>BBa_K3384311</partinfo> in pRS426, this construct expressed GFP. Then the activity of the bybrid promoter can be quantitatively measured through flow cytometer. As is shown in Fig. 1, the UAS<sub>TEF</sub>-pTEF promoter strength was expanded beyond the pTEF by 1.2-fold.
+When assembled with GFP <partinfo>BBa_K3112009</partinfo>, and <partinfo>BBa_K3384311</partinfo> in pRS426, this construct expressed GFP. Then the activity of the bybrid promoter can be quantitatively measured through flow cytometer. As is shown in '''Fig. 1''', the UAS<sub>TEF</sub>-pTEF promoter strength was expanded beyond the pTEF by 1.2-fold.
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 <p> </p>