Difference between revisions of "Part:BBa K3989010"
Line 7: Line 7:
 
[[File:ClyA_in_omp8_OMV.jpeg|500px]]
 
[[File:ClyA_in_omp8_OMV.jpeg|500px]]
  
−
Figure 1. ClyA protein exists in outer membrane vesicles of E. coli omp8 strain.
+
Figure 1. ClyA protein with or without different PAMPs exists in outer membrane vesicles of E. coli omp8 strain.
  
 
[[File:omp8 OMVs on col-0.jpeg|500px]]
 
[[File:omp8 OMVs on col-0.jpeg|500px]]
−
Figure 2
+
Figure 2.
  
 
[[File:tolB OMVs on col-0.jpeg|500px]]
 
[[File:tolB OMVs on col-0.jpeg|500px]]

Revision as of 15:56, 16 October 2021


plant immune elicitor flg22 fused with ClyA for OMV display

In this construct, outer membrane-associated protein ClyA is used to display the plant immune elicitor flg22 to trigger plant immune response.

ClyA in omp8 OMV.jpeg

Figure 1. ClyA protein with or without different PAMPs exists in outer membrane vesicles of E. coli omp8 strain.

Omp8 OMVs on col-0.jpeg Figure 2.

TolB OMVs on col-0.jpeg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1105
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1105
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1105
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1105
    Illegal NgoMIV site found at 1099
    Illegal AgeI site found at 1063
  • 1000
    COMPATIBLE WITH RFC[1000]