Difference between revisions of "Part:BBa K3753003"

(→‎Characterization)
Line 4: Line 4:
 
p<em>TEF2</em> is a strong constitutive promoter in <em>Saccharomyces cerevisiae</em>, which controls the transcription of its downstream sequence coding for translational elongation factor EF-1 &alpha;.
 
p<em>TEF2</em> is a strong constitutive promoter in <em>Saccharomyces cerevisiae</em>, which controls the transcription of its downstream sequence coding for translational elongation factor EF-1 &alpha;.
  
 +
===Characterization===
 +
 +
<br>
 +
<p> </p>
 +
[[File:Gold.png|width='100%' valign='top'| |center|thumb|550px|''<b>Fig.1</b> ]]
 +
<br>
 +
When assembled with GFP BBa_K3112009, and CYC1 terminator BBa_K3384311 in pRS426, pTEF2 regulated the expression of GFP.
 +
Three pTEF2-based synthetic hybrid promoters were designed to enhance promoter activity. For detailed information, please visit UASCLB-pTEF BBa_K3753011, UASCIT-pTEF BBa_K3753012, UASTEF-pTEF BBa_K3753013.
 +
<br>
 +
<p> </p>
 +
<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:09, 16 October 2021

pTEF2

pTEF2 is a strong constitutive promoter in Saccharomyces cerevisiae, which controls the transcription of its downstream sequence coding for translational elongation factor EF-1 α.

Characterization


Fig.1


When assembled with GFP BBa_K3112009, and CYC1 terminator BBa_K3384311 in pRS426, pTEF2 regulated the expression of GFP. Three pTEF2-based synthetic hybrid promoters were designed to enhance promoter activity. For detailed information, please visit UASCLB-pTEF BBa_K3753011, UASCIT-pTEF BBa_K3753012, UASTEF-pTEF BBa_K3753013.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]