Difference between revisions of "Part:BBa K3893027"

Revision as of 14:10, 16 October 2021

AHL induced expression of Lysis protein.

Figure 1. SBOL Visual representation of the part

AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893026, is a component of the intermediate part [1] to assemble the release system device ([2]) of our Agrobactory 593. It is used to express the lysis protein phiX174 when AHL (bound to to LuxR) is present, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

BBa_K2656003: the R0062 inducible pLux promoter in its Golden Braid compatible version
BBa_K2656010: the B0032 weak ribosome biding site in its Golden Braid compatible version
BBa_K2656015: the phiX174 lysis protein
Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656015 is the lysis gene from the enterobacteria phage phiX174 coding sequence. The lysis gene E of coliphage PhiX174 encodes a 91-amino acid (aa) residue protein of approximately 10 kDa that mediates inhibition of peptidoglycan biosynthesis of Gram-negative bacteria membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.
-This composite part, together with [https://parts.igem.org/Part:BBa_K3893026 K3893026], is a component of the intermediate part [https://parts.igem.org/Part:BBa_K3893028]  to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030]) of our Agrobactory 593. It is used to express the lysis protein Phi174 when AHL (bound to to LuxR) is present, a fundamental piece of our population oscillator. release device.
+This composite part, together with [https://parts.igem.org/Part:BBa_K3893026 K3893026], is a component of the intermediate part [https://parts.igem.org/Part:BBa_K3893028]  to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030]) of our Agrobactory 593. It is used to express the lysis protein phiX174 when AHL (bound to to LuxR) is present, a fundamental piece of our population oscillator. release device.
 This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
@@ Line 18: / Line 18: @@
 <li></html>[https://parts.igem.org/Part:BBa_K2656010 BBa_K2656010]: the [https://parts.igem.org/Part:BBa_B0032 B0032] weak ribosome biding site in its Golden Braid compatible version <html></li>
-<li></html> [https://parts.igem.org/Part:BBa_K2656015 BBa_K2656015]: the Pho174 lysis protein <html></li>
+<li></html> [https://parts.igem.org/Part:BBa_K2656015 BBa_K2656015]: the phiX174 lysis protein <html></li>
 <li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>